FIGURE 7.

S32T mutation abolishes Prdx6 targeting to lamellar bodies in mouse lungs. LAMP-1 immunofluorescence (green) is used as a marker for lysosome-like organelles (left panels). Immunofluorescence for Prdx6 expression (red) is shown in the middle panels. Co-localization is indicated by yellow fluorescence in the merged images (right panels). All three panels for each condition are of the identical field. The area in the upper LAMP-1 panel for each condition that is enclosed by a box is enlarged as shown in the lower panels. A, immunofluorescence of wild type lungs. B, immunofluorescence of S32T-Prdx6 lungs.