TABLE 1.

aiPLA₂ activity and kinetic constants for recombinant WT and Ser-32 mutant Prdx6

Kinetic constants were calculated from the data presented in Fig. 3. Values for activity are mean ± S.E. for n = 3 independent experiments.

	aiPLA2 activity	Km	Vmax
	nmol/min/mg protein	μm	mol/min/mg protein
WT	104 ± 3	336	134
S32T	100 ± 3	343	133
S32A	0.9 ± 0.3a

^a p < 0.05 versus wild type or S32A.