TABLE 2.

GSH peroxidase activity in recombinant protein and in lysates of MPMVEC expressing WT or Ser-32 mutant Prdx6 delivered by lentivirus infection

Prdx6 null MPMVEC were infected with lentivirus expressing wild type or mutant human Prdx6 or vector alone. Values are mean ± S.E. for n = 3 independent experiments.

	Recombinant protein		MPMVEC lysates
	H2O2	PLPCOOH	H2O2	PLPCOOH
	μmol/min/mg protein		nmol/min/mg protein
Vector	−	−	131 ± 3.6a	1.0 ± 0.2a
Wild type	4.2 ± 0.4	4.8 ± 0.4	242 ± 1.0	12.9 ± 0.7
S32T-Prdx6	3.8 ± 0.2	4.7 ± 0.2	234 ± 9.1	12.5 ± 0.3
S32A-Prdx6	3.0 ± 0.2	0a	237 ± 13.5	3.4 ± 0.5a

^a p < 0.05 versus corresponding WT.