TABLE 2.

Summary of parameters calculated for various experiments of tubulin dimer dissociation

Each isotherm was modeled individually (locally), i.e. not a global analysis.

Parametera	SV			FA
	Tubulin dilution	Tubulin-488 dilution	Tubulin-550 dilution	Tubulin-488 titration	Tubulin-345 titration	Tubulin-345 dilution
monomer, s20, w or anisotropy	3.0 (<1.5–3.1)	3.2 (2.9–3.4)	3.2 (3.0–3.2)	0.243 (0.238–0.247)	0.094 (0.091–0.097)	0.087 (0.084–0.089)
dimer, s20, w or anisotropy	5.9 (5.8–6.2)	6.0 (5.8–6.4)	5.7 (5.6–5.8)	0.280 (0.271–0.299)	0.153 (0.145–0.165)	0.167 (0.164–0.171)
Kd, nm	39 (15–74)	40 (23–69)	65 (51–83)	347 (71–1650)	265 (57–1250)	125 (59–262)
koff, s−1						3.1 × 10−3 (2.7–3.6)

^a Values are the best-fits with 68% confidence intervals in parentheses.