FIGURE 5.
Pet54 does not affect the levels of Pet309 and Mss51 proteins and shows a mild genetic interaction with Mss51. A sample of 10 and 50 μg of mitochondrial protein from strains expressing the Mss51–3xHA (A) or Pet309–3xHA (B) as well as the untagged versions of these genes were separated by SDS-PAGE and analyzed by Western blotting. The membranes were probed with antibodies anti-HA and anti-citrate synthase (CS) as a loading control. C, cells carrying the COX1(1512)::ARG8m construct were transformed with empty plasmid (vector) or with the indicated genes cloned on YEp352 plasmid. The transformants were spotted on synthetic complete medium lacking (−ARG) or containing (+ARG) arginine. 10-Fold serial dilutions were grown for 3 days at 30 °C. The black arrow indicates the processing site for the pre-Arg8 matrix-targeting signal.