TABLE 1.
Strains used in this study
All of these strains are congenic or isogenic to D273-10B. Mitochondrial genotypes are shown in parenthesis. ΔΣai represents an intronless COX1 gene. cox1Δ::ARG8m-1 has a replacement of the COX1 3′-UTR by the COX2 3′-UTR. cox1Δ::ARG8m-2 has a replacement of the COX1 5′-UTR by the COX2 5′-UTR (40).
| Strain | Nuclear (mitochondrial) genotype | Reference/Source |
|---|---|---|
| XPM10b | Matα, arg8::hisG, leu2-3,112, lys2, ura3-52 (ρ+,cox1 Δ::ARG8m) | Ref. 20 |
| XPM11 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112 (ρ+, COX1(1–512)::ARG8m) | Ref. 20 |
| XPM48 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δmss51::LEU2 (ρ+, COX1(1–512)::ARG8m) | Ref. 20 |
| XPM171 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112 (ρ+, cox1Δ::ARG8m, cox2Δ::COX1, COX2)a | Ref. 20 |
| XPM182 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet111::URA3 (ρ+, cox1Δ::ARG8m, cox2Δ::COX1, COX2)a | Ref. 20 |
| XPM201 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112 (ρ+, ΔΣai) | Ref. 16 |
| XPM209 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112 (ρ+, ΔΣai, COX1ΔC15) | Ref. 16 |
| XPM295 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51–3xHA (ρ+, ΔΣai) | Ref. 16 |
| XPM298 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA (ρ+, ΔΣai, COX1ΔC15) | Ref. 16 |
| XPM315 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4 (ρ+, COX1(1–512)::ARG8m) | Ref. 16 |
| XPM316 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4 (ρ+, ΔΣai) | Ref. 16 |
| XPM317 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4 (ρ+, ΔΣai, COX1ΔC15) | Ref. 16 |
| YC61 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcox6::KANMX4 (ρ+, COX1(1–512)::ARG8m) | Ref. 16 |
| YC75 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcox14::URA3 (ρ+, ΔΣai) | Ref. 16 |
| YC76 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcox14::URA3 (ρ+, ΔΣai, COX1ΔC15) | Ref. 16 |
| YC77 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4 (ρ+, ΔΣai), Δcox14::URA3 | This work |
| YC78 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4, cox14Δ::URA3 (ρ+, ΔΣai, COX1ΔC15) | Ref. 16 |
| YC100 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δpet54::KANMX (ρ+, ΔΣai) | This work |
| YC102 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet494::KANMX (ρ+, ΔΣai) | This work |
| YC 103 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet494::KANMX (ρ+, ΔΣai, COX1ΔC15) | This work |
| YC104 | Matα, arg8::hisG, leu2-3,112, lys2, ura3-52, Δpet494::KANMX (ρ+, cox1Δ::ARG8m) | This work |
| YC105 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet494::KANMX (ρ+, cox1Δ::ARG8m, cox2Δ::COX1, COX2)a | This work |
| YC106 | Matα, arg8::hisG, leu2-3,112, lys2, ura3-52, Δpet122::KANMX (ρ+, cox1Δ::ARG8m) | This work |
| YC109 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, PET54-3xMyc (ρ+, ΔΣai) | This work |
| YC112 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcoa3::LEU2 (ρ+, ΔΣai) | This work |
| YC113 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcoa3::LEU2 (ρ+, ΔΣai, COX1ΔC15) | This work |
| YC114 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4, Δcoa3::LEU2 (ρ+, ΔΣai, COX1ΔC15) | This work |
| YC118 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4, Δcoa3::LEU2 (ρ+, ΔΣai) | This work |
| LSR13 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet122::KANMX4 (ρ+, ΔΣai) | Ref. 16 |
| LSR28 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet122::KANMX4 (ρ+, ΔΣai, COX1ΔC15) | Ref. 16 |
| LSR33 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX (ρ+, cox1Δ::ARG8m, cox2Δ::COX1, COX2)a | This work |
| LSR39 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcox14::URA3 (ρ+, COX1(1–512)::ARG8m) | This work |
| LSR43 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcox6::KANMM (ρ+, COX1(1–512)::ARG8m) | Ref. 16 |
| LSR44 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet122::KANMX (ρ+, cox1Δ::ARG8m, cox2Δ::COX1, COX2)a | This work |
| SB5 | Matα, ade2, lys2, Δura3, PET309::3xHA (ρ+) | Ref. 60 |
| JPM18 | Matα, arg8::hisG, leu2-3,112, lys2, ura3-52, Δpet54::KANMX (ρ+, cox1Δ::ARG8m) | This work |
| JPM21 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet309::URA3 (ρ+, COX1(1–512)::ARG8m) | This work |
| JPM23 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δcox14::URA3, Δpet54::KANMX (ρ+, COX1(1–512)::ARG8m) | This work |
| JPM30 | Matα, ade2, lys2, Δura3, PET309-3xHA, Δpet54::KANMX (ρ+) | This work |
| JPM40 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δcox14::URA3 (ρ+, ΔΣai) | This work |
| JPM42 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δpet54::KANMX, Δcox14::URA3 (ρ+, ΔΣai) | This work |
| JPM43 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet111::URA3, Δpet54::KANMX (ρ+, cox1Δ::ARG8m, cox2Δ::COX1, COX2), | This work |
| JPM44 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, PET54-3xMyc, Δmss51::LEU2 (ρ+, ΔΣai) | This work |
| JPM49 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δpet122::KANMX (ρ+, ΔΣai) | This work |
| JPM50 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA,Δcox11::KANMX (ρ+, ΔΣai) | This work |
| JPM52 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, PET54-3xMyc, Δpet494::URA3 (ρ+, ΔΣai) | This work |
| JPM53 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, PET54-3xMyc, Δpet309::URA3 (ρ+, ΔΣai) | This work |
| JPM55 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δpet122::KANMX (ρ+, ΔΣai) | This work |
| JPM56 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δpet54::KANMX (ρ+, ΔΣai, COX1ΔC15) | This work |
| JPM57 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, MSS51-3xHA, Δpet122::KANMX (ρ+, ΔΣai, COX1ΔC15) | This work |
| XPM330 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112 (ρ+, cox1Δ::ARG8m-1, cox2Δ::COX1, COX2)a | This work |
| RGV125 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX (ρ+, cox1Δ::ARG8m-1, cox2Δ::COX1, COX2)a | This work |
| XPM329 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112 (ρ+, cox1Δ::ARG8m-2, cox2Δ::COX1, COX2)a | This work |
| RGV124 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX (ρ+, cox1Δ::ARG8m-2, cox2Δ::COX1, COX2)a | This work |
| AGG81 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, LEU2::YIplac128-mss51F199I (ρ+, ΔΣai) | This work |
| AGG82 | Matα, lys2, arg8::hisG, ura3-52, leu2-3, 112, Δpet54::KANMX4 LEU2::YIplac128-mss51F199I (ρ+, ΔΣai) | This work |
a Ectopic insertion of the chimeric COX1 gene upstream of the COX2 gene.