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. 2016 Feb 24;291(17):9371–9381. doi: 10.1074/jbc.M115.705251

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Tor1 and Gad8 are required for efficient binding of the MBF complex to its target promoters. A, loading of Cdc10 on cdc22, cdt2, and cdc18 promoters was measured by ChIP analysis of chromatin extracts isolated from wild-type (WT), Δgad8, or Δtor1 cells carrying chromosomally tagged Cdc10-MYC. The level of binding is quantified on anti-MYC-immunoprecipitated DNA. Each value is the mean of at least three independent assays, and the error bars indicate S.D. B, loading of Res1 on cdc22, cdt2, and cdc18 promoters was measured by ChIP analysis of cells carrying chromosomally tagged Res1-MYC. C, loading of Res2 on cdc22, cdt2, and cdc18 promoters was measured by ChIP analysis of cells carrying chromosomally tagged Res2-MYC. D, Tor1 and Gad8 are required for efficient binding of the MBF complex under DNA replication stress. Loading of Res1 on cdc22 and cdc18 promoters was analyzed by ChIP analysis of untreated cells (−) or cells treated with 12 mm HU for 3 h (+).