Table 1. Characteristics of 32 rabies virus samples from dogs, Mali, 2006–2013* .

Virus	GenBank accession no.	Sample ID no.	Region	Quantitative RT-PCR Ct	Subgroup of Africa 2 lineage
RV01	KP976113	420/2006	Bamako	28.51	G
RV04	KP976114	345/2007	Bamako	30.19	H
RV05	KP976130	352/2007	Bamako	25.35	G
RV06	KP976125	58/2008	Bamako	24.09	G
RV09	KP976126	146/2008	Bamako	27.51	G
RV10	KP976122	154/2008	Ségou	25.75	G
RV11	KP976124	167/2008	Koulikoro	23.97	G
RV14	NA	259/2008	Bamako	31.59	H
RV15	KP976123	261/2008	Ségou	26.14	G
RV19	NA	530/2008	Bamako	27.85	G
RV20	KP976117	003/2009	Bamako	32.82	H
RV22	NA	69/2009	Bamako	26.15	H
RV27	NA	118/2009	Bamako	32.30	H
RV44	KP976129	587/2009	Bamako	26.22	G
RV50	NA	19/11/2010	Bamako	27.90	G
RV51	NA	42/2010	Bamako	28.59	G
RV56	NA	171/2010	Koulikoro	22.20	G
RV57	KP976121	176/2010	Gao	21.90	F
RV60	NA	221/2010	Bamako	24.60	H
RV67	NA	603/2010	Bamako	21.30	H
RV68	NA	137/2011	Bamako	20.80	H
RV70	KP976119	149/2011	Bamako	21.70	H
RV79	NA	339/2011	Bamako	24.90	G
RV81	KP976127	357/2011	Bamako	34,20	G
RV84	NA	480/2011	Bamako	21.20	G
RV87	KP976116	612/2011	Bamako	22.50	H
RV88	KP976120	628/2011	Koulikoro	21.70	H
RV89	NA	674/2011	Bamako	20.20	H
RV90	KP976118	688/2011	Bamako	30.80	H
RV93	KP976115	223/2012	Bamako	23.20	H
RV95	NA	366/2012	Bamako	21.00	G
RV96	KP976128	100/2013	Bamako	29.00	G
*A fluorescent antibody test was conducted as described by Dean et al. (5). For each tested sample, test paper was impregnated with 100 μL of 10% brain suspension and subjected to molecular biological analysis. Of 100 samples tested, 32 showed positive results by this test. A conventional hemi-nested reverse transcription PCR (RT-PCR) was performed with rabies virus primers JW12–JW6 as described (9). All samples showed positive results by this test. A quantitative RT-PCR was performed with rabies primers JW12–N165-146 (10). This PCR detected >100 RNA copies/µL. The coefficient of determination (R2) was 0.999, the Y intercept was of 36.65, and efficiency was 99%. Samples in bold (n = 15) had duplicate sequences and were not subjected to phylogenetic analysis. ID, identification; Ct, cycle threshold; NA, not available.