Table 1. Primers and probes used in real-time PCR for Ehrlichia and Anaplasma species, United States*.

Name	Sequence, 5′ → 3′	Concentration, nmol/L	Species detected
Primer 1	GCATTACTCACCCGTCTGCCACT	250	–
Primer 2	CAAGCCTAACACATGCAAGTCGAACG	1,000	–
Probe 1	MGB-FAM-AGGT†T†ATAA†GCA†ATTGTCC-EDQ	200	E. chaffenisis
Probe 2	MGB-(AP642)-AAGCTATA†GGCA†GT†TA†TCC-EDQ	200	E. muris–like agent
Probe 3	MGB-(AP593)-GGCTATA†A†ATA†A†TTGT†CCG-EDQ	200	E. canis
Probe 4	MGB-(AP593)-CTATTTA†GGA†A†TTGT†T†C-EDQ	200	E. ewingii
Probe 5	MGB-(AP525)-AAAGAAT†A†A†TCCGTTCG-EDQ	200	A. phagocytophilum

*–, these primers are specific for 5 organisms; MGB, minor groove binder; FAM, 6-carboxyfluorescein; EDQ, Eclipse Dark Quencher (Epoch Biosciences Inc., Bothell, WA, USA).
†Use of Super A, T, or G (Epoch Biosciences Inc.).