Figure 5.

Rab GTPases recruited to PI(3,4,5)P₃-enriched early phagosomes after closure. RAW 264.7 macrophages were cotransfected to express GFP-tagged Rab5a, Rab5b, Rab5c, Rab11a, Rab14, Rab20, or Rab33a and mCherry-Akt-PH. Macrophages were exposed to IgG-opsonized sRBCs (IgG-sRBC) for 5 min and fixed. Exposed IgG-sRBC surfaces were probed with Alexa405 anti-human IgG. Representative images show examples of GFP-Rab recruitment to phagocytic cups/phagosomes. Insets correspond to arrows indicating representative phagocytic cups/phagosomes. Phagocytic cups and phagosomes counted per classification are expressed as percentages and recorded in the Venn diagrams on the right. At least 40 phagosomes from 5 or more cells were quantified.