Figure 7.

Rab GTPases not recruited during early stages of phagocytosis. RAW 264.7 macrophages were cotransfected to express GFP-tagged Rab22a, Rab29, Rab30, Rab32, Rab40, or Rab42 and mCherry-Akt-PH. Macrophages were exposed to IgG-sRBCs for 5 min and fixed with 4% paraformaldehyde to halt phagocytosis. Exposed IgG-sRBC surfaces were probed with Alexa405 anti-human IgG. Representative images of each Rab were chosen based on no observable GFP-Rab recruitment to phagocytic cups or phagosomes, detected by enrichment of mCherry-Akt-PH. Phagocytic cups and phagosomes counted per classification are expressed as percentages and recorded in the Venn diagrams on the right. At least 40 phagosomes from 5 different cells were quantified.