Figure 9.

Summary of sequential Rab recruitment and enrichment during early stages of phagocytosis. A schematic diagram summarizing the relationships between Rab and phosphoinositide enrichment on membranes throughout phagocytosis. Rab35 and Rab13 are present on the PI(4,5)P₂-enriched plasma membrane and are rapidly recruited to phagocytic cups, overlapping with enrichment of PI(3,4,5)P₃, while Rab13 persists after cup closure. Rab8a, Rab8b, and Rab27a are locally enriched on the nascent phagocytic cup, mirroring the association and dissociation of PI(3,4,5)P₃ on the phagosomal membrane. This is followed by Rab10 in concert with PI(3,4,5)P₃ enrichment and dissociation. Rab31 is recruited just prior to cup closure and it persists during phagosome maturation. Rab5a, Rab5b, Rab5c, and Rab20 are distinctly enriched on the phagosome after dissociation of PI(3,4,5)P₃ and all persist during maturation.