Figure 2. Conformational changes in the complex of CrPV-IRES with the 80S ribosome and eEF2.
(A) Overview of the complex with the large ribosomal subunit (60S) depicted in blue, the small ribosomal subunit (40S) yellow, the CrPV-IRES green and the eEF2 in red. CrPV-IRES and eEF2 are represented as cartoon models inside the corresponding semi-transparent computed molecular surfaces. (B) Superposition of low-pass filtered (8Å) volumes from a front view of the 40S showing an additional ~3º rotation induced in the small subunit by eEF2 binding (yellow) when compared with the rotated state without eEF2 (violet, EMDB-2603). No swivelling of the 40S-head was observed. (C) Additional displacement of the L1 stalk induced by the binding of eEF2. The RNA component of the L1 stalk in the present structure with eEF2 (blue), the rotated state of the complex without eEF2 (violet; PDB 4V92), and in a complex with tRNAs in the hybrid state (orange; PDB-ID 4V9H).
Figure 2—figure supplement 1. EM image processing workflow for the 80S/CrPV-IRES/eEF2-GDPCP data.
