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. 2016 May 10;8:107. doi: 10.3389/fnagi.2016.00107

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Copyright © 2016 Decock, Stanga, Octave, Dewachter, Smith, Constantinescu and Kienlen-Campard.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of oligomeric bands and measurement of Aβ in the extracellular medium of cells expressing C99 and C42. (A) Culture media of CHO cells expressing APP, C99, and C42 were analyzed by Western blotting using the W0-2 antibody. The ∼30 kDa oligomer band (^∗) detected in culture media -similar to the one observed in cell lysates- is indicated by an arrow. (B) Soluble monomeric Aβ38, Aβ40, and Aβ42 were quantified by ECLIA in the culture media of transfected cells. Values (means ± SEM) given in pg/ml are representative of three independent experiments (n = 3 in each experiment). ^∗∗∗p < 0.001, ^∗p < 0.05, as compared to control (mock-transfected cells).