Table 2.

Hemolytic grades against human erythrocytes, cytotoxicity (IC₅₀) against rat cardiomyocytes, HEK293 cells, and HeLa cells, and half-life times in mouse serum determined for Api137 and the four new apidaecin analogs.

	Hemolytic grade [%]		IC50 on cell viability [g/L]			Half-life time mouse serum (min)
	0.1 g/L	0.6 g/L	Rat cardiomyocytes	HEK293	HeLa
Api137*	0.4 ± 0.5	1.0 ± 0.2	>0.6	>0.6	>0.6	345
Api793	1.3 ± 0.4	1.6 ± 0.5	>0.6	0.64 ± 0.05	0.64 ± 0.15	246
Api794	1.7 ± 0.2	1.9 ± 0.3	>0.6	0.28 ± 0.03	0.23 ± 0.09	311
Api795	−0.8 ± 0.4	0.0 ± 0.7	>0.6	>0.6	>0.6	354
Api796	−0.8 ± 0.2	−0.1 ± 0.8	>0.6	>0.6	>0.6	249

^*Data for Api137 were already published (Berthold et al., 2013; Bluhm et al., 2015).