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. 2016 May 10;7:167. doi: 10.3389/fimmu.2016.00167

Figure 1.

Figure 1

Schematic drawings of the constructs included in the study. (A) Drawing of the designed p62/SQSTM1-containing vaccine construct. The 3-prime end of DNA of p62 is connected to the antigenic unit via a linker region (L) encoding the amino acids RSTGLSGL. The DNA construct was subcloned into the pBudCE4.1 vector, and expression of the gene was driven by human cytomegalovirus immediate-early promoter (pCMV). Arrow heads indicate unique sites for restriction enzymes. Phox/Bem 1p (PB1), LC3-interacting region (LIR), and ubiquitin-associated domain (UBA) encode some of the functional domains of p62. (B) Overview of all the constructs that were included in the study, (i) p62–Gagp24 (Gagp24 isolate BH10), (ii) Gagp24, (iii) Gagp24 fused to mCherry, and (iv) p62 fused to mCherry. mCherry was included to have the possibility to track p62 and Gagp24 by live-cell fluorescence microscopy and was subcloned into the antigenic unit. All constructs were subcloned into the pBudCE4.1 vector downstream of pCMV.