Figure 7.

Mouse responsiveness to number of Gagp24-derived peptide pools. The graph presents the number of peptide pools that each immunized BALB/c mouse responded to with IFNγ⁺ T cells. The mice were immunized once with an intradermal injection on the left and right flank with 25 μl of 12.5 μg DNA plasmid encoding Gagp24 or p62–Gagp24 before electroporation. Splenocytes were harvested after 5, 7, or 18 weeks and subjected to IFNγ-ELISpot for enumeration of Gagp24-reactive T cell responses toward five different Gagp24-derived peptide pools described in the legend of Figure 6. In the analysis, 20 IFNγ⁺ SFC/106 splenocytes were set as threshold, and only responses toward a given peptide pool that were above the threshold were included. Gagp24, n = 16 mice; p62–Gagp24, n = 14 mice. The P-value was calculated by Mann–Whitney test.