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. 2016 May 10;7:687. doi: 10.3389/fmicb.2016.00687

Figure 1.

Figure 1

The effects of rnc gene on exopolysaccharide synthesis and biofilm morphology in S. mutans. The anthrone assay was used to determine glucan productions in UA159, Smurnc, and Smurnc+ biofilms at 24 h. WSGs (A) and WIGs (B) were both calculated. The Smurnc strain decreased the amount of glucans, whereas the Smurnc+ strain increased the amount. The error bars represent standard deviation values. Asterisks indicate significant differences (P < 0.05). (C) Representative three-dimensional images of production and distribution of exopolysaccharide matrix in the biofilm architecture. Mature biofilms (24 h) formed by S. mutans UA159 and rnc mutant strains in the presence of 1% (wt/vol) sucrose by CLSM. Images were taken at 63× magnification. (D) SEM observation of the architecture of S. mutans mature biofilms (24 h). Images of biofilm structures formed by UA159 and rnc mutant strains were taken at 20,000× magnification.