Fig. 8.

Sequence-based and chemical shift-based predictions from NMRFAM-SPARKY of the secondary structure of the protein brazzein (PDB ID: 2LY5, BMRB ID: 16215). The illustration was made by NDPPlot. a Sequence-based prediction by PSIPRED (two-letter-code PP) could not predict secondary structures correctly and the probabilities are not confident. b Chemical shift-based prediction by PECAN (two-letter-code n6). c Secondary structures from PDB deposited structure. The PECAN prediction matched perfectly with the secondary structural elements determined from the structure