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. 2016 May;186(5):1258–1266. doi: 10.1016/j.ajpath.2015.12.021

Figure 4.

Figure 4

Macrophage colony-stimulating factor (M-CSF) in IL-1β– or tumor necrosis factor (TNF)-α–stimulated first-trimester decidual cells (FTDCs) enhances phagocytic capacity of macrophages and down-regulates signal-regulatory protein (SIRP)α expression on macrophages. Macrophages (red) treated with conditioned medium (CM) from control (EM; A), IL-1β (EMI; B), or TNF-α (EMT; C) stimulates FTDCs with or without anti-human M-CSF antibody in the presence of PKH67-labeled HTR-derived apoptotic bodies (green; D and E). Macrophages engulfing apoptotic bodies are indicated by arrows. F and G: The phagocytosis index was calculated as follows: the percentage of CD11b+ phagocytosing macrophages × mean value of green fluorescence measured by flow cytometry. SIRPα expression on macrophages was assessed by quantitative RT-PCR (H) and enzyme-linked immunosorbent assay (I). Results are expressed as means ± SEM (F–I). n = 3 (F–I). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.005 versus EM; †P < 0.05, ††P < 0.01 versus EMI and EMT. Scale bar = 50 μm (A–E). Original magnification, ×200 (A–E). α-M, anti-M-CSF; HTR, HTR-8/SVNeo cells.