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. 2016 May;186(5):1387–1402. doi: 10.1016/j.ajpath.2016.01.010

Figure 8.

Figure 8

Polymorphonuclear (PMN) transendothelial migration (TEM) is inhibited in endothelial cells expressing vascular endothelial (VE)-cadherin double mutant (DMT). Endothelial cells were transduced with VE-cadherin wild-type (WT) green fluorescent protein (GFP) adenovirus or VE-cadherin DMT GFP. A and B: Time lapse of VE-cadherin GFP (top rows) and merged differential interference contrast and VE-cadherin GFP images (bottom rows) of PMN cells in the process of transmigration. A: In cells expressing VE-cadherin WT GFP, PMN cells locomote to a nearby cell-cell junction (white arrow, time = 00:00, merge). A VE-cadherin gap forms (white arrows, time = 03:40, top row and merge) just as the PMN cell is in the act of transmigrating (white arrows, time = 03:40, merge). TEM occurs within 5 minutes, and VE-cadherin gap reseals shortly after. B: In contrast, cells that express VE-cadherin DMT fail to form a VE-cadherin gap (white arrows, top row) despite the presence of a PMN crawling along a cell-cell junction for up to 20 minutes (white arrows, merge). These images are representative of 100 PMN interactions observed in at least five different experiments. Scale bars: 10 μm.