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SDS-PAGE of proteins from cells and YCPs expressing U65-GFP (34.1 kDa, arrow). For each set, the first lane shows total proteins, the second is 16,000 ×g pellet, and the third is 16,000 ×g supernatant. Lanes 1–3, cells. Lanes 4–6, YCPs made by ficin hydrolysis. Lanes 7–9, YCPs made by hydrolysis at 45°C, pH 11.5 to 11.3.
