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Antiapoptotic effect of ADM on LPS-induced apoptosis in Leydig cells. Leydig cells were treated with ADM (100 nM) with or without LY294002 for 2 h prior to a 12-hour treatment with LPS (1 μg/mL). Cell apoptosis was assessed using TUNEL labeling and DAPI staining; DNA fragmentation was analyzed using ELISA detection (OD, optical density). Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.
