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Effect of ADM on the LPS-induced gene expressions of Bcl-2, Bax, caspase-3, and PARP. Leydig cells were treated with ADM (100 nM) or ADM (100 nM) + LY294002 (10 μmol/L) for 2 h before a 12-hour treatment with LPS (1 μg/mL). The normalized levels of gene expression are expressed as ratios of the copy number of the mRNA and that of β-actin cDNA. Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.
