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. 2016 Apr 26;2016:7201549. doi: 10.1155/2016/7201549

Figure 7.

Figure 7

Effect of ADM on LPS-induced inhibition of Akt phosphorylation. Leydig cells were treated with ADM (100 nM) or ADM (100 nM) + LY294002 (10 μmol/L) for 2 h before a 12-hour treatment with LPS (1 μg/mL). β-actin was used as internal reference, and p-Akt levels were normalized to total Akt levels. Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.