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. 2016 May 10;6:25385. doi: 10.1038/srep25385

Figure 1. Effect of lnc-RI knockdown on cell growth.

Figure 1

(a) Schematic of the lnc-RI locus. (b) Expression profile of lnc-RI in multiple cell types. (c) Effect of siRNAs on HEK293 cells and HeLa cells. HEK293 cells or HeLa cells were transfected with lnc-RI RNAi-1, lnc-RI RNAi-2, or Ctrl RNAi. Real time-PCR analysis was performed at 48 h after transfection. (d) Effect of the knockdown of lnc-RI on the morphology of HEK293 at 48 h post-transfection (Bar, 50 μm). (e) Effect of the knockdown of lnc-RI on growth of HEK293 and HeLa cells. HEK293 and HeLa cells were transfected with lnc-RI RNAi -2 and the numbers of cells were counted using Cell Counting Kit-8 at 1, 2, 3, and 4 d after transfection. (f) Effect of the knockdown of lnc-RI on colony formation of HeLa cells. HeLa cells were transfected with plasmid expressing lnc-RI shRNA. Cells were cultured with G418 (1000 ng/mL) for 14 days and numbers of colony were analysed. *P-value < 0.05, **P-value < 0.01.