Figure 2. Muc5ac from “non-secretor” Fut2-null mice displays enrichment in Lewis a glycan structures.

Wild-type and Fut2-null mice total gastric mucosa protein lysates were used for evaluation of (a) Muc5ac (45M1) and (b) Le^a (SPM279) expression by Western blotting. Labels (1) and (2) represent protein lysates from two independent mice samples. (c) G-sepharose beads coupled with Muc5ac recognizing antibody 45M1 were incubated with wild-type and Fut2-null mice gastric mucosa protein extracts. Proteins that bound to the beads were analyzed by Western blotting with an anti -Le^a antibody.