Table 1. The kinetic characterisation of AF-Est2 using pNP-esters with varying acyl chain length and pNP-benzoate as substrates.

	kcat (s−1)	KM (μM)	kcat/K M (s−1 μM−1)
C2	21.5 ± 0.2	620 ± 10	0.0349 ± 0.0007
C3	47.8 ± 0.5	254 ± 8	0.189 ± 0.006
C4	55.5 ± 0.5	92 ± 3	0.61 ± 0.02
C5	58.9 ± 0.2	19 ± 1	3.1 ± 0.2
C8	46.0 ± 0.3	120 ± 10	0.37 ± 0.03
C12	0	N/A	0
Benzoate	0	N/A	0

No activity was detected for substrates with an acyl chain length of twelve carbons or more.