Table 2. Primer sequence information for the LAMP reaction.
| Label | Len | Tm (°C) | 5′dG | 3′dG | G/C | Sequence(5′ → 3′) |
|---|---|---|---|---|---|---|
| F3 | 20 | 59.53 | −5.00 | −6.24 | 0.50 | CTTGGTATTCCATGGGGCAT |
| B3 | 21 | 59.49 | −3.66 | −4.06 | 0.43 | ACTTTTGGACGTCGTCGTTAT |
| FIP | 40 | GAGGCGAGACAAACACCCAACA-GCCTGTTCGAGCGTCATT | ||||
| BIP | 41 | CGCCTTAAAACAATTGGCAGCCG-GAGTGCAAAGCGCGAGAT | ||||
| LF | 22 | 61.06 | −5.85 | −4.74 | 0.45 | CCAAGCAAAGCTTGAAGGTACA |
| LB | 23 | 65.65 | −5.30 | −6.57 | 0.52 | GCGTATTGATTTCGGAGCGCAGT |
The A. rabiei target amplification fragment length using the outer primers F3 with B3 was 183 bp.