Figure 8. Effects of the AtDPG1 mutation on the expression of nuclear-encoded photosynthetic genes.

(a) The expression levels of nuclear-encoded genes that are critical for chlorophyll biosynthesis (HEMA1, CHLH, CHL27, CHLP, PORB, PORC, CAO, and CLA1). (b) The expression levels of nuclear-encoded photosynthetic genes (OE23, OE33, PSAN, PSBW, LHCB1, LHCA4, LHCB4, and RBCS). (c) The expression levels of nuclear-encoded GLK transcription factor genes (AtGLK1 and AtGLK2). The terms dpg1-I and dpg1-II refer to the leaves that were albino or pale-green in 21-day-old and 35-day-old dpg1 mutants, respectively. The transcript level of each gene was set to 1.0 in the leaves from a 21-d-old wild type plant. The bar graphs show the relative expression levels, as determined by the comparative C_T method and normalized with the expression of the ACTIN2 gene. The error bars indicate the interval delimited by 2^{–(ΔΔCT ± SD)}. The asterisks indicate ΔC_T values significantly different from those of control in a Mann–Whitney U-test (P < 0.05).