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. 2016 May 10;6:25675. doi: 10.1038/srep25675

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Copyright © 2016, Macmillan Publishers Limited

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All recombinant strains were constructed in the adh1Δ strain. Fermentation was performed in anaerobic glass tubes in YPAD media with 20 g/L glucose under micro-anaerobic condition as previously described¹⁸. HOM3*: HOM3 mutant encoding HOM3^G1355A ²⁷; LEU4s: truncated LEU4 that starts at +91 ATG³³. Error bars indicate standard deviations of three biological replicates. A student’s t-test was used for statistical analysis of the n-butanol production in triplicates. Solid pentagram (★) indicates a result that was significantly different compared to the reference adh1Δ strain (p < 0.05); empty pentagram (☆) indicates a result that was significantly different compared to the Hom3m strain (p < 0.05).