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. 2016 May 10;6:25675. doi: 10.1038/srep25675

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Copyright © 2016, Macmillan Publishers Limited

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(A) The synergistic pathway for n-Butanol production via the endogenous threonine pathway and a introduced CimA mediated pathway. Single and double arrows represent single and multiple enzymatic steps respectively; red arrows represent heterologous pathways; overexpressed genes are marked with red colour. KDCs: keto-acid decarboxylases; ADHs: alcohol dehydrogenases. (B) Production of n-butanol in strains expressing the optimized threonine pathway (THRm) and the synergistic pathways with CimA from different species. Error bars indicate standard deviations of three biological replicates. A student’s t-test was used for statistical analysis of the n-butanol production in triplicates. Solid pentagram (★) indicates a result that was significantly different compared to the THRm strain (p < 0.05).