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. 2016 May 10;6:25680. doi: 10.1038/srep25680

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Copyright © 2016, Macmillan Publishers Limited

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(A) SDS-PAGE profile of the affinity-purified BPL proteins and the AccB87substrate (B). TLC-based analyses of BPL activity. Minus means no addition of either BPL protein or AccB87 substrate protein and plus denotes addition of AccB87. Products of the BPL reaction were separated on TLC and visualized using phosphor-imaging. Abbreviations: M, protein marker (Bio-Rad); ec, E. coli; LL, L. lactis; BPL, biotin protein ligase.