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. 2016 May 10;6:25680. doi: 10.1038/srep25680

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(A) MALDI-TOF determination of the molecular weight of the un-biotinylated AccB87 polypeptide. The calculated mass for AccB87 is 9335.5~9336.6 and the expected mass for biotinoyl-AccB87 is 9562.3~9562.9. (B) The formation of the biotinylated AccB87 by E. coli BirA. (C) Biotinylation of AccB87 by L. lactis BirA1. (D) Biotinylation of AccB87 by L. lactis BirA2. Designations: minus denotes no addition of BirA enzyme, plus denotes addition of the BirA protein. Abbreviations: ec, E. coli; LL, L. lactis.