. Author manuscript; available in PMC: 2016 May 10.

Published in final edited form as: Nat Protoc. 2015 Oct 22;10(11):1820–1841. doi: 10.1038/nprot.2015.115

TABLE 1.

Allelic exchange vectors for Pseudomonas aeruginosa

Vector^a, ^b	Description and pertinent features	Accession No^c	Reference
Restriction cloning
pEX18Ap^a, ^b, ^d	Ap^r/Cb^roriT, sacB, lacZα, MCS from pUC18	AF004910	²⁰
pEX18Gm^a, ^b	Gm^roriT, sacB, lacZα, MCS from pUC18	AF047518	²⁰
pEX18Tc^a, ^b	Tc^roriT, sacB, lacZα, MCS from pUC18	AF047519	²⁰
pEX19Gm^b	Gm^roriT, sacB, lacZα, MCS from pUC19	KM887142 ^c	^20,99
pEXG2	Gm^rmob, sacB, lacZα	KM887143 ^c	¹⁰⁰
pEX19EYFP	Gm^roriT, sacB, lacZα, MCS from pUC19, yfp	---	⁸¹
pEX19ECFP	Gm^roriT, sacB, lacZα, MCS from pUC19, cfp	---	⁸¹
Saccharomyces cerevisiae-based recombineering
pMQ30	Gm^roriT, sacB, lacZα, URA3	DQ230317	²⁶
pMQ75	Gm^roriT, sacB, lacZα, URA3	DQ230319	⁷⁴
Gateway® cloning - donor vectors
pDONRPEX18Ap^a	Cm^r and Ap^r/Cb^r, pEX18Ap with Gateway donor site	KM880129 ^c	This study
pDONRPEX18Gm^a	Cm^r and Gm^r, pEX18Gm with Gateway donor site	KM880128 ^c	This study
pDONRPEX18Tc^a	Cm^r and Tc^r, pEX18Tc with Gateway donor site	KM880130 ^c	This study
pDONRX	Cm^r and Gm^r, pMK2010 with Gateway donor site	---	³⁰
Gateway® cloning - destination vectors^e
pEX18ApGW^a, ^b	Cm^r and Ap^r/Cb^r, pEX18Ap with Gateway destination site	AY928469	²⁷
pEX18GmGW^a	Cm^r and Gm^r, pEX18Gm with Gateway destination site	KM880127 ^c	²⁹
pEXG2GW	Cm^r and Gm^r, pEXG2 with Gateway destination site	---	³⁰

Plasmids available by request to Joe J. Harrison (jjharris@ucalgary.ca).

Plasmids available by request to Herbert Schweizer (hschweizer@ufl.edu).

Sequencing primers used to obtain DNA sequence deposited in Genbank are listed in Table S1.

Orignially named pEX18T.

Destination vectors can be designated either by a ‘pDEST’ prefix or ‘GW’ suffix. Both designations are equivalent and utilized in the literature. Antibiotic selection (E. coli/P. aeruginosa): Ap, ampicillin; Cb, carbenicillin; Cm, chloramphenicol, Gm, gentamicin; Tc, tetracycline. oriT, origin of transfer; sacB, levansucrase; lacZα, α-peptide of β-galactosidase.