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. Author manuscript; available in PMC: 2016 May 10.
Published in final edited form as: Nat Protoc. 2015 Oct 22;10(11):1820–1841. doi: 10.1038/nprot.2015.115
Cycle Description Temperature (°C) Time (s)
1 Initial denature 98 30
2 – 35 Denature 98 10
Anneal Gradient (55 – 72) 15
Extend 72 20 per 1 kb of the amplicon
36 Final extend 72 300
37 Hold 10 infinite