Cycle | Description | Temperature (°C) | Time (s) |
---|---|---|---|
1 | Initial denature | 98 | 30 |
2 – 35 | Denature | 98 | 10 |
Anneal | Gradient (55 – 72) | 15 | |
Extend | 72 | 20 per 1 kb of the amplicon | |
36 | Final extend | 72 | 300 |
37 | Hold | 10 | infinite |
Cycle | Description | Temperature (°C) | Time (s) |
---|---|---|---|
1 | Initial denature | 98 | 30 |
2 – 35 | Denature | 98 | 10 |
Anneal | Gradient (55 – 72) | 15 | |
Extend | 72 | 20 per 1 kb of the amplicon | |
36 | Final extend | 72 | 300 |
37 | Hold | 10 | infinite |