Component | Volume (µl) | ||
---|---|---|---|
1 reaction | 5 reactions | 10 reactions | |
HF buffer (5 ×) | 4.00 | 20.0 | 40.0 |
dNTPs (10 mM) | 0.40 | 2.0 | 4.0 |
DMSO, 100% | 0.60 | 3.0 | 6.0 |
F primer (100 µM) | 0.05 | 0.25 | 0.50 |
R primer (100 µM) | 0.05 | 0.25 | 0.50 |
DNA fragments* | x + y + (z) | 5[x + y + (z)] | 10[x + y + (z)] |
Ultrapure water | 14.7 – [x + y + (z)] | 73.5 – 5[x + y + (z)] | 147 – 10[x + y + (z)] |
Phusion® DNA polymerase | 0.20 | 1.0 | 2.0 |
Total volume | 20.00 | 100.0 | 200.0 |