Component	Volume (µl)
	1 reaction	5 reactions	10 reactions
Green GoTaq buffer (5×)	4.00	20.0	40.0
MgCl2 (25mM)	2.00	10.0	20.0
dNTPs (10 mM)	0.40	2.00	4.00
DMSO, 100%	0.60	3.00	6.00
M13F primer (100 µM)	0.05	0.25	0.50
M13R primer (100 µM)	0.05	0.25	0.50
Ultrapure water	11.8	59.0	118
GoTaq® polymerase	0.10	0.50	1.00
Total volume	19.00	95.0	190.0