Cycle | Description | Temperature (°C) | Time (s) |
---|---|---|---|
1 | Initial denature | 95 | 180 |
2 – 30 | Denature | 95 | 10 |
Anneal* | Tm | 30 | |
Extend | 72 | 60 per 1 kb of the amplicon | |
31 | Final extend | 72 | 300 |
32 | Hold | 10 | infinite |
Cycle | Description | Temperature (°C) | Time (s) |
---|---|---|---|
1 | Initial denature | 95 | 180 |
2 – 30 | Denature | 95 | 10 |
Anneal* | Tm | 30 | |
Extend | 72 | 60 per 1 kb of the amplicon | |
31 | Final extend | 72 | 300 |
32 | Hold | 10 | infinite |