| Component | Volume (µl) | ||
|---|---|---|---|
| 1 reaction | 5 reactions | 10 reactions | |
| Green GoTaq buffer (5×) | 6.0 | 30.0 | 60.0 |
| MgCl2 (25mM) | 3.0 | 15.0 | 30.0 |
| dNTPs (10 mM) | 0.6 | 3.0 | 6.0 |
| DMSO, 100% | 0.9 | 4.5 | 9.0 |
| Seq-F primer (100 µM) | 0.1 | 0.5 | 1.0 |
| Seq-R primer (100 µM) | 0.1 | 0.5 | 1.0 |
| Ultrapure water | 18.15 | 90.75 | 181.5 |
| GoTaq® polymerase | 0.15 | 0.75 | 1.5 |
| Total volume | 29.0 | 145.0 | 290.0 |
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