Skip to main content
. 2015 May 27;33(8):2469–2482. doi: 10.1002/stem.2051

Figure 2.

Figure 2

Characterization of photoreceptor precursor (PPr) surface biomarkers on three‐dimensional embryonic stem cell (ESC) retinal cultures. (A, B): Day 12 optic vesicle neuroepithelium showing Vsx2 (green, A) and Pax6 (red, B) positive retinal progenitor cells. (C, D): Day 28 retinal neuroepithelium regions containing Rhodopsin (green, C) and Recoverin (red, D) positive ESC‐derived photoreceptors. (E, F): Immunohistochemical analysis showing a small number of cells positive for Rod α‐transducin (E) and Peripherin 2 (F) at day 28 of culture. High magnification inserts highlight expression pattern of these markers. (G): Quantitative real‐time PCR analysis of ESC retinal cultures demonstrating the expression of PPr biomarker panel over time in culture. (H): Expression of the PPr biomarker panel on day 26 fluorescent‐activated cell‐sorted Rhop.GFP+ ESC‐derived rods. (I): Immunohistochemical analysis for CD73, CD24, CD133, and CD47 (red), and Recoverin and Rhodopsin (green) on cryosections of ESC‐retinal differentiations at day 12, 28, and 36 of culture. Scale bars: 25 µm. Abbreviation: ES, embryonic stem cell (undifferentiated); PCR, polymerase chain reaction.