Fig. 5.

Potential predictive biomarkers for cytotoxic effect of mitotic drugs. a Evaluation of recurrent genetic alteration in the studied 16 TNBC cell lines. The heat map in panel a represents the average cytotoxic response of CDK-, mitotic-, mTOR- and proteasome inhibitors whereas in panel b heat map represents recurrent mutations in each of the cell lines. The analysis failed to link the cytotoxic responses to any mutation detected. c Heat map highlighting/differentiating mitotic inhibitor-sensitive and insensitive cell lines based on cytotoxicity exhibited by 5 different mitotic drugs. The dose response curves (right) illustrate the effects of the representative mitotic inhibitor dinaciclib on cell viability and toxicity in two individual cell lines. d Box plots showing the differential expression of protein and phosphoprotein levels (from Daemen et al. [15]) between mitotic inhibitor sensitive and insensitive cell lines