Fig. 7.

Assessing both cell viability and cell death is necessary to evaluate drug combination effect. Seven different concentrations of two different drugs were combined in 8 × 8 matrix format, in which blue matrix shows % inhibition in viability whereas red matrix shows % cell death (toxicity), and adjacent 7 × 7 matrix represents the combined effects of the drugs (Bliss synergy score), in which pink indicates synergy and green antagonism. a In MDA-MB-231 and DU4475 combination of dactolisib and trametinib showed additive effect in enhancing viability inactivation whereas dactolisib antagonized the cytotoxicity of trametinib in both cell lines. b In DU4475 combination of trametinib with iniparib and ponatinib enhanced both viability inactivation and cell death effect but it is more pronounced in cytotoxicity readout than in the viability readout