Table 3.

Differentially expressed genes in fetal intestine by maternal high fat intake and validated by qPCR

		Fold changeb
Gene symbola	cDNA Microarray	qPCR	P value
ACTA2	1.66	1.10	0.246
SULT1E1	2.59	1.88	0.002
SOD2	1.51	1.75	0.036
BMP7	−1.59	−1.09	0.722
CD40	−2.00	−1.74	0.116
CD74	−1.49	−1.36	0.029
CD8A	−2.44	−1.85	0.049
GK	−1.64	−1.33	0.041
HSPAIL	−2.50	−1.60	0.027
PIK3R5	−1.56	−1.07	0.644
PSTPIP1	−1.85	−1.48	0.083
SLA-1	−1.49	−1.45	0.097
SLA-DQA1	−1.85	−1.79	0.003
SLA-DRB1	−1.52	−1.33	0.136
STAT2	−1.69	−1.18	0.125
TGF-β	−1.52	−1.19	0.296
THY1	−1.52	−1.29	0.118

^aGenes were selected on the basis of their crucial role on regulating intestinal immune response (i.e.SLA-DRB1,SLA-DQA,HSPA1L,CD74,CD40), colorectal cancer (i.e.TGF-β,PIK3R5), signal transduction (i.e. PSTPIP1,BMP7,STAT2) and metabolism (i.e.GK, SULT1E1). These genes by DNA microarray were all significantly regulated (P < 0.05, at least 1.5 fold change)

^bThe fold change was based on the ratio of HF group to CON group, n = 4 subpools/group