Fig. 1.

The proportions of naive and memory CD4⁺ T cell subsets in HEU and HUU children at different ages. Cells were stained anti CD3 PE, anti CD4 PerCP, anti CD8 APC-H7, anti CD45RA FITC and anti CCR7 APC monoclonal antibodies. a A gate was drawn on CD4⁻ CD8⁻(non T cells) and CD4⁺ CD8⁻ (CD4⁺ T cells) populations and plotted on CD45RA FITC against CCR7 APC. Using quadrant statistics, distinct populations of naïve (CD45RA⁺ CCR7⁺), central memory (CD45RA⁻CCR7⁺), effector memory (CD45RA⁻ CCR7⁻), and terminally differentiated (CD45RA⁺ CCR7⁻) CD4⁺ T cells were identified. Median proportions of Naïve CD4⁺ (b), central memory CD4⁺ (c), effector memory CD4⁺ (d), and terminally differentiated CD4⁺T lymphocytes (e) were determined in peripheral blood of HEU and HUU children at 6, 12 and 18 months. X-axis represents age of children in months. Horizontal bars represent medians