Table 2.
Identified proteins in plasma by nLC-MS/MS
| Spot Nr | Protein | SwissProt | Mw/pI | Score | Sequence coverage [%] | Nr of unique peptides |
|---|---|---|---|---|---|---|
| 1 | Alpha-1-antitrypsin | P01009 | 46878 / 5.37 | 194 | 21 | 7 |
| 2 | Alpha-1-antitrypsin | 474 | 33 | 12 | ||
| 3 | Alpha-1-antitrypsin | 382 | 32 | 15 | ||
| 4 | Complement factor B | P00751 | 86847 / 6.67 | 225 | 12 | 10 |
| 5 | Complement factor B | 266 | 16 | 12 | ||
| 6 | Complement factor B | 339 | 15 | 15 | ||
| 7 | Complement factor B | 252 | 15 | 14 | ||
| 8 | Alpha-1B-Glycoprotein | P04217 | 54790 / 5.56 | 51 | 1 | 6 |
| 9 | Alpha-1B-Glycoprotein | 108 | 7 | 3 | ||
| 10 | Alpha-1B-Glycoprotein | 142 | 12 | 5 | ||
| 11 | Serotransferrin | P02787 | 79294 / 6.81 | 414 | 19 | 13 |
| 12 | Serotransferrin | 988 | 52 | 31 | ||
| 13 | Serotransferrin | 889 | 42 | 28 | ||
| 14 | Serotransferrin | 1107 | 49 | 31 | ||
| 15 | Serotransferrin | 1078 | 49 | 31 | ||
| 16 | N-acetylmuramoyl-L-alanine amidase | Q96PD5 | 62748 / 7.25 | 31 | 1 | 1 |
| 17 | N-acetylmuramoyl-L-alanine amidase | 67 | 3 | 2 | ||
| 18 | N-acetylmuramoyl-L-alanine amidase | 98 | 3 | 1 | ||
| 19 | Hemopexin | P02790 | 52385 / 6.55 | 212 | 14 | 6 |
| 20 | Hemopexin | 306 | 23 | 11 | ||
| 21 | Hemopexin | 314 | 26 | 12 | ||
| 22 | Hemopexin | 329 | 25 | 11 | ||
| 23 | Hemopexin | 298 | 15 | 10 | ||
| 24 | Hemopexin | 277 | 20 | 9 | ||
| 25 | Kininogen | P01042 | 72996 / 6.34 | 321 | 11 | 6 |
| 26 | Kininogen | 308 | 9 | 5 | ||
| 27 | Kininogen | 203 | 10 | 6 | ||
| 28 | Hemopexin | P02790 | 52385 / 6.55 | 266 | 20 | 8 |
| 29 | Hemopexin | 49 | 2 | 1 | ||
| 30 | Beta-2-Glycoprotein 1 | P02749 | 39584 / 8.34 | 172 | 16 | 5 |
| 31 | Kininogen | P01042 | 72996 / 6.34 | 127 | 8 | 4 |
| 32 | Kininogen | 275 | 17 | 8 | ||
| 33 | Kininogen | 236 | 13 | 8 | ||
| 34 | Kininogen | 254 | 15 | 8 | ||
| 35 | Alpha-1-antitrypsin | P01009 | 46878 / 5.37 | 962 | 45 | 17 |
| 36 | Alpha-1-antitrypsin | 730 | 43 | 18 | ||
| 37 | Alpha-1-antitrypsin | 1009 | 47 | 17 | ||
| 38 | Alpha-1-antitrypsin | 509 | 42 | 16 | ||
| 39 | Vitamin D-binding protein | P02774 | 54526 / 5.40 | 476 | 36 | 16 |
| 40 | Alpha-1-antitrypsin | P01009 | 46878 / 5.37 | 620 | 51 | 19 |
| 41 | Vitamin D-binding protein | P02774 | 54526 / 5.40 | 381 | 25 | 11 |
| 42 | Antithrombin III | P01008 | 53025 / 6.32 | 333 | 25 | 10 |
| 43 | Leucine-rich alpha-2-glycoprotein | P02750 | 38382 / 6.45 | 224 | 17 | 6 |
| 44 | Leucine-rich alpha-2-glycoprotein | 131 | 14 | 5 | ||
| 45 | Leucine-rich alpha-2-glycoprotein | 134 | 17 | 4 | ||
| 46 | Haptoglobin | P00738 | 45861 / 6.13 | 302 | 21 | 9 |
| 47 | Haptoglobin | 273 | 26 | 10 | ||
| 48 | Haptoglobin | 296 | 27 | 10 | ||
| 49 | Zinc-Alpha-2-glycoprotein | P25311 | 34465 / 5.71 | 223 | 17 | 7 |
| 50 | Zinc-Alpha-2-glycoprotein | 226 | 24 | 8 | ||
| 51 | Clusterin | P10909 | 53031 / 5.89 | 219 | 17 | 7 |
| 52 | Clusterin | 203 | 14 | 7 | ||
| 53 | Clusterin | 331 | 22 | 9 | ||
| 54 | ApoA1 | P02647 | 30759 / 5.56 | 419 | 44 | 13 |
| 55 | ApoA1 | 775 | 62 | 16 | ||
| 56 | ApoA1 | 4287 | 53 | 14 | ||
| 57 | ApoA1 | 850 | 48 | 8 | ||
| 58 | Haptoglobin | P00738 | 45861/6.13 | 2 | ||
| 59 | Haptoglobin | 2 | ||||
| 60 | Transthyretin | P02766 | 15887/ 5.5 | 4357 | 69.4 | 11 |
| 61 | Transthyretin | 8483 | 64.6 | 7 | ||
| 62 | ApoCIII | P02656 | 8800/4.7 | 65 | 4 | |
| 63 | ApoCIII | 65 | 4 | |||
| 64 | ApoAII | P02652 | 9300/6.5 | 56 | 5 | |
| 65 | ApoCII | P02655 | 8900/4.7 | 96 | 6 | |
| 66 | Alpha-2-HS-Glycoprotein | P02765 | 39325/5,4 | 60245 | 22.9 | 9 |
| 67 | Alpha-2-HS-Glycoprotein | 60245 | 20.4 | 8 | ||
| 68 | Alpha-2-HS-Glycoprotein | 60245 | 20.4 | 8 | ||
| 69 | Alpha-2-HS-Glycoprotein | 60245 | 20.4 | 8 | ||
| 70 | Haptoglobin | P00738 | 45861/6.13 | 14986 | 24.1 | 11 |
| 71 | Haptoglobin | 12798 | 23.2 | 8 | ||
| 72 | Haptoglobin | 12728 | 21.9 | 8 | ||
| 73 | Haptoglobin | 60393 | 28.3 | 11 | ||
| 74 | Haptoglobin | 12798 | 23.2 | 8 | ||
| 75 | Haptoglobin | 12728 | 21.9 | 8 |
The spot numbers refer to marked protein spots in Fig. 1