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. 2016 May 9;15:78. doi: 10.1186/s12934-016-0472-0

Table 3.

Oligonucleotides used for polymerase chain reaction

Gene Oligonucleotide
Name Sequence
syatprr2 (with His6-tag) syatprr2_tag_fw 5′-GGGTTTCATATGAAAGAGACTAACTTCGGCG-3′
syatprr2_tag_rev 5′-CCGCTCGAGTCAGTGGTGATGATGGTGATGACCGCCGACGAAAATTTTCAG-3′
syfiplr (with His6-tag) syfiplr_tag_fw 5′-GGGTTTCATATGGGCAAATCCAAAGTTCTG-3′
syfiplr_tag_rev 5′-CCGCTCGAGTCAGTGGTGATGATGG-3′
syfiplr (no tag) syfiplr_fw 5′-GGGTTTCATATGGGCAAATCCAAAGTTCTG-3′
syfiplr_rev 5′-CCGCTCGAGTCAAACATAGCGTTTAAGGTATTCTTCAAC-3′