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Oligonucleotides used for polymerase chain reaction
| Gene | Oligonucleotide | |
|---|---|---|
| Name | Sequence | |
| syatprr2 (with His6-tag) | syatprr2_tag_fw | 5′-GGGTTTCATATGAAAGAGACTAACTTCGGCG-3′ |
| syatprr2_tag_rev | 5′-CCGCTCGAGTCAGTGGTGATGATGGTGATGACCGCCGACGAAAATTTTCAG-3′ | |
| syfiplr (with His6-tag) | syfiplr_tag_fw | 5′-GGGTTTCATATGGGCAAATCCAAAGTTCTG-3′ |
| syfiplr_tag_rev | 5′-CCGCTCGAGTCAGTGGTGATGATGG-3′ | |
| syfiplr (no tag) | syfiplr_fw | 5′-GGGTTTCATATGGGCAAATCCAAAGTTCTG-3′ |
| syfiplr_rev | 5′-CCGCTCGAGTCAAACATAGCGTTTAAGGTATTCTTCAAC-3′ | |
