Table 2.

Prevalence of tick-borne pathogens as detected by PCR in 76 cervids and 65 wild boars from Centre and southern Portugal

Idependent variable/category	Cervus elaphus & Dama dama					Sus scrofa
	No. (%)	Anaplasma spp.		Theileria spp.		No. (%)	Anaplasma spp.		Theileria spp.
		% of positive	95 % CI	% of positive	95 % CI		% of positive	95 % CI	% of positive	95 % CI
District	76	ND*		ND*		65	ND*		ND*
Castelo Branco	27 (35.5)	59.3a	38.8–77.6	44.4	25.5–64.7	4 (6.2)	0	0–60.2	0.0	0.0–60.2
Portalegre	1 (1.3)	100	2.5–100	100	2.5–100	15 (23.1)	0	0–21.8	0.0	0.0–21.8
Lisboa	3 (3.9)	66.7	9.4–99.2	66.7	9.4–99.2	16 (24.6)	0	0–20.6	0.0	0.0–20.6
Évora	–	–	–	–	–	15 (23.1)	0	0–21.8	0.0	0.0–21.8
Beja	45 (59.2)	31.1a	18.2–46.7	42.2	27.7–57.9	15 (23.1)	13.3	1.66–40.5	20.0	4.3–48.1
Age	61	P = 0.309		P = 0.607c		62	P = 1		P = 0.545
Adult	50 (82.0)	40.0	26.4–54.8	46.0	31.8–60.7	42 (67.7)	4.8	0.6–16.2	7.1	1.5–19.5
Young	11 (18.0)	18.2	2.3–51.8	54.5	23.4–83.3	20 (32.3)	0	0–16.8	0.0	0.0–16.8
Gender	69	P = 0.618b		P = 0.025d		63	P = 0.493		P = 1
Female	37 (53.6)	37.8	22.5–55.2	32.4	18.0–49.8	45 (71.4)	2.2	0.1–11.8	4.4	0.5–15.2
Male	32 (46.4)	43.8	26.4–62.3	59.4	40.7–76.3	18 (28.6)	5.6	0.1–27.3	5.6	0.1–27.3
Total	76	43.4	32.1–55.3	44.7	33.3–56.6	65	3.1	0.4–10.7	4.6	1.0–12.9

^a χ ² = 5.50, df = 1, P = 0.019

^b χ ² = 0.25, df = 1

^c χ ² = 0.26, df = 1

^d χ ² = 5.03, df = 1

ND* Statistically significant difference(s) not confirmed after pairwise comparisons