Table 1.
Targeted organisms and list of primers used in this study
| Target organism | Primer | Sequence | Fragment length (bp) | Reference |
|---|---|---|---|---|
|
Ehrlichia spp./
Anaplasma spp. |
E.c 16S-fwd E.c 16S-rev |
TCGCTATTAGATGAGCCTACGT GAGTCTGGACCGTATCTCAGT |
123 | [4] |
| Anaplasmataceae | ECB ECC |
CGTATTACCGCGGCTGCTGGCA AGAACGAACGCTGGCGGCAAGC |
500 | [5] |
| Ehrlichia canis | ECAN5 HE3 |
CAATTATTTATAGCCTCTGGCTATAGGA ATAGGGAAGATAATGACGGTACCTATA |
400 | [5] |
| Anaplasma platys | ApysF ApysR |
GTCGAACGGATTTTTGTCGT TAGATCACCGCCTTGGTAGG |
200 | [5] |
|
Babesia spp./ Hepatozoon spp. |
Piroplasmid-F Piroplasmid-R |
CCAGCAGCCGCGGTAATTC CTTTCGCAGTAGTTYGTCTTTAACAAATCT | 400 | [6] |
| Babesia spp. | Babesia18S-F Babesia18S-R |
CCGTGCTAATTGTAGGGCTAATACA GCTTGAAACACTCTARTTTTCTCAAAG | 551 | [7] |
| Hepatozoon spp. | Hepatozoon18S-F Hepatozoon18S-R |
GGTAATTCTAGAGCTAATACATGAGC ACAATAAAGTAAAAAACAYTTCAAAG |
574 | [7] |
bp, base pairs